tris glycine buffer Search Results


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List of antibodies used in this study
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Image Search Results


List of antibodies used in this study

Journal: The Journal of Biological Chemistry

Article Title: Endoplasmic Reticulum Stress-associated Cone Photoreceptor Degeneration in Cyclic Nucleotide-gated Channel Deficiency *

doi: 10.1074/jbc.M112.342220

Figure Lengend Snippet: List of antibodies used in this study

Article Snippet: All other reagents were purchased from Sigma, Bio-Rad, and Invitrogen. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Provider Catalog no. Dilutions used in immunoblotting M-opsin Dr. Cheryl Craft (Keck School of Medicine) 1:2000 Cone arrestin (CAR) Dr. Cheryl Craft (Keck School of Medicine) 1:2000 S-opsin Dr. Muna Naash (University of Oklahoma Health Sciences Center) 1:1000 CNGA3 Custom antibody generated by YenZym Antibodies, LLC (Ref. 25 ) 1:250 Gnat2 Santa Cruz Biotechnology sc-390 1:500 GADD 153 (CHOP-10) Santa Cruz Biotechnology sc-575 1:100 Phospho-eIF2α Cell Signaling Technology 3398 1:500 Phospho-IP 3 R Cell Signaling Technology 3760 1:250 Caspase-7 Cell Signaling Technology 9492 1:250 Caspase-12 Cell Signaling Technology 2202 1:250 Endo G Cell Signaling Technology 4969 1:250 AIF Cell Signaling Technology 4642 1:500 Cytochrome c Cell Signaling Technology 4272 1:250 Caspase-9 Cell Signaling Technology 9504 1:250 Caspase-3 Cell Signaling Technology 9661 1:250 Calpain II Cell Signaling Technology 2539 1:500 Bcl-2 Epitomic 1017-1 1:250 Bcl-2-x L Epitomic 1018-1 1:250 Calpain I Abcam ab28258 1:500 Grp78/BiP Abcam ab21685 1:500 β-Actin Abcam ab-6276 1:2000 Acetyl-histone H3 (H3) Upstate Cell Signaling Solution 07-540 1:2000 TATA binding protein Thermo Scientific, Inc. MA1-10883 1:500 Open in a separate window List of antibodies used in this study Recordings of Electroretinograms (ERG) Full-field ERG recordings were carried out as described previously ( 16 ).

Techniques: Western Blot, CRAfT Assay, Generated, Binding Assay

Enhanced levels of phospho-IP3R and calpains in CNGA3−/−/Nrl−/− and CNGB3−/−/Nrl−/− retinas. The expression levels of phospho-IP3R, calpain I, and calpain II were examined in CNGA3−/−/Nrl−/−, CNGB3−/−/Nrl−/−, and Nrl−/− mice at P30. A, shown are representative images of the Western blot detections. Total retinal protein lysate was used for the detections, and actin was used as loading control. B, densitometric analysis of the relative expression levels of phospho-IP3R (left panel) and calpain II (right panel) in CNGA3−/−/Nrl−/−, CNGB3−/−/Nrl−/−, and Nrl−/− retinas. Data are represented as means ± S.E. of measurements from three to four independent experiments using retinas from four to five mice. Unpaired Student's t test was used for determination of the significance (*, p < 0.05). C, shown are representative images of the Western blot detections of Bcl-2 and Bcl-x in the mouse retina. Total retinal protein lysate was used, and actin was used as a loading control.

Journal: The Journal of Biological Chemistry

Article Title: Endoplasmic Reticulum Stress-associated Cone Photoreceptor Degeneration in Cyclic Nucleotide-gated Channel Deficiency *

doi: 10.1074/jbc.M112.342220

Figure Lengend Snippet: Enhanced levels of phospho-IP3R and calpains in CNGA3−/−/Nrl−/− and CNGB3−/−/Nrl−/− retinas. The expression levels of phospho-IP3R, calpain I, and calpain II were examined in CNGA3−/−/Nrl−/−, CNGB3−/−/Nrl−/−, and Nrl−/− mice at P30. A, shown are representative images of the Western blot detections. Total retinal protein lysate was used for the detections, and actin was used as loading control. B, densitometric analysis of the relative expression levels of phospho-IP3R (left panel) and calpain II (right panel) in CNGA3−/−/Nrl−/−, CNGB3−/−/Nrl−/−, and Nrl−/− retinas. Data are represented as means ± S.E. of measurements from three to four independent experiments using retinas from four to five mice. Unpaired Student's t test was used for determination of the significance (*, p < 0.05). C, shown are representative images of the Western blot detections of Bcl-2 and Bcl-x in the mouse retina. Total retinal protein lysate was used, and actin was used as a loading control.

Article Snippet: All other reagents were purchased from Sigma, Bio-Rad, and Invitrogen. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Provider Catalog no. Dilutions used in immunoblotting M-opsin Dr. Cheryl Craft (Keck School of Medicine) 1:2000 Cone arrestin (CAR) Dr. Cheryl Craft (Keck School of Medicine) 1:2000 S-opsin Dr. Muna Naash (University of Oklahoma Health Sciences Center) 1:1000 CNGA3 Custom antibody generated by YenZym Antibodies, LLC (Ref. 25 ) 1:250 Gnat2 Santa Cruz Biotechnology sc-390 1:500 GADD 153 (CHOP-10) Santa Cruz Biotechnology sc-575 1:100 Phospho-eIF2α Cell Signaling Technology 3398 1:500 Phospho-IP 3 R Cell Signaling Technology 3760 1:250 Caspase-7 Cell Signaling Technology 9492 1:250 Caspase-12 Cell Signaling Technology 2202 1:250 Endo G Cell Signaling Technology 4969 1:250 AIF Cell Signaling Technology 4642 1:500 Cytochrome c Cell Signaling Technology 4272 1:250 Caspase-9 Cell Signaling Technology 9504 1:250 Caspase-3 Cell Signaling Technology 9661 1:250 Calpain II Cell Signaling Technology 2539 1:500 Bcl-2 Epitomic 1017-1 1:250 Bcl-2-x L Epitomic 1018-1 1:250 Calpain I Abcam ab28258 1:500 Grp78/BiP Abcam ab21685 1:500 β-Actin Abcam ab-6276 1:2000 Acetyl-histone H3 (H3) Upstate Cell Signaling Solution 07-540 1:2000 TATA binding protein Thermo Scientific, Inc. MA1-10883 1:500 Open in a separate window List of antibodies used in this study Recordings of Electroretinograms (ERG) Full-field ERG recordings were carried out as described previously ( 16 ).

Techniques: Expressing, Western Blot, Control